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Image Search Results
Journal: Cell Death & Disease
Article Title: Ablation of beta subunit of protein kinase CK2 in mouse oocytes causes follicle atresia and premature ovarian failure
doi: 10.1038/s41419-018-0505-1
Figure Lengend Snippet: a Immunoblotting analysis of DNA damage response signaling in ovaries of Ck2β fl/fl and Ck2β fl/fl ;GCre + mice at 2 weeks after birth. The ovary lysates were collected at least from three mice of each genotype and immunoblotted for γH2AX, H2AX, p-CHK2 (T68), CHK2, p63, p-p53 (S15), p53 and β-actin. Level of β-actin was detected as internal control. Each experiment was repeated at least 2–3 times. Molecular mass is given in kilo Daltons. b Immunoblotting analysis of the expression of p53 and p63 in ovaries of Ck2β fl/fl and Ck2β fl/fl ;GCre + at 4 weeks after birth. The ovary lysates were obtained from at least three mice of each genotype and immunoblotted for p63, p-p53 (S15), p53 and β-actin. Level of β-actin was used as internal control. Each experiment was repeated at least three times. Molecular mass is given in kilo Daltons. c MVH and γH2AX immunofluorescent staining of 2-week-old ovarian sections from Ck2β fl/fl and Ck2β fl/fl ;GCre + mice. Green: γH2AX; Red: MVH; Blue: DAPI. At least three mice of each genotype were used in this assay. Scale bar: 50 μm
Article Snippet: Rabbit monoclonal anti-CK2β antibody (AJ1128b; ABGENT); mouse monoclonal anti-β-actin antibody (sc-47778; Santa Cruz); mouse monoclonal anti-MVH antibody (ab27591; abcam); rabbit monoclonal anti-p-Akt (S473) antibody (4046; Cell Signaling Technology, Inc.); rabbit monoclonal anti-p-Akt (T308) (13038; Cell Signaling Technology, Inc.); rabbit monoclonal anti-p-Akt (S129) (ab133458; abcam); rabbit monoclonal anti-AKT1/2/3 antibody (ab32505; abcam); rabbit monoclonal anti-PTEN antibody (9559; Cell Signaling Technology, Inc.); rabbit polyclonal anti-p-TSC2 (S1387) antibody (5584; Cell Signaling Technology, Inc.); rabbit monoclonal anti-TSC2 antibody (4308; Cell Signaling Technology, Inc.); rabbit monoclonal anti-p-mTOR (S2448) antibody (5536; Cell Signaling Technology, Inc.); rabbit monoclonal anti-p-S6K (T389) antibody (9234; Cell Signaling Technology, Inc.); rabbit monoclonal anti-p-rpS6 (S240/244) antibody (5364; Cell Signaling Technology, Inc.); rabbit polyclonal anti-GSK3α/β (S21/9) antibody (9331; Cell Signaling Technology, Inc.); rabbit monoclonal anti-GSK3α/β antibody (5676; Cell Signaling Technology, Inc.); rabbit polyclonal anti-p-FOXO1 (T24)/FOXO3a (T32) antibody (9464; Cell Signaling Technology, Inc.); rabbit monoclonal anti-FOXO1 antibody (2880; Cell Signaling Technology, Inc.); rabbit monoclonal anti-γH2AX antibody (9718; Cell Signaling Technology, Inc.); rabbit monoclonal anti-H2AX antibody (7631; Cell Signaling Technology, Inc.);
Techniques: Western Blot, Expressing, Staining
Journal: OncoTargets and therapy
Article Title: RSF-1 siRNA Enhances Tumor Radiosensitivity in Cervical Cancer via Enhanced DNA Damage, Cell Cycle Redistribution, and Promotion of Apoptosis
doi: 10.2147/OTT.S246632
Figure Lengend Snippet: RSF-1 siRNA impairs ATM/ATR signaling pathway activated by radiation. ( A ) HeLa and SiHa cells transfected with siRNA-NC or siRNA-RSF-1 were irradiated with 0, 4 and 10Gy, and the ATM/ATR pathway proteins were examined by Western blot. ( B ) The relative gray values of p-ATM, p-ATR, p-CHK1 and p-CHK2 were calculated and graphed for each group. The experiments were repeated three times independently. *** P <0.01, ** P <0.01, * P <0.05 as compared with the siRNA-NC group.
Article Snippet: Anti-ATR, -ATM, -CHK1, -
Techniques: Transfection, Irradiation, Western Blot